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DOI: 10.1055/s-0038-1651249
Modification of the Fibrin Agar Plate for Measurements of the Components of the Fibrinolytic System
II. Measurement of Intrinsic Plasminogen Activator and Its Inhibitor (on Type II Fibrin Agar Plate)Publication History
Publication Date:
27 June 2018 (online)
Summary
The optimal conditions for plasminogen activation by intrinsic activator (IA) were found on Type II human fibrin agar plates made with .04 M pH 8.5 NH4 acetate buffer.
Intrinsic activation inhibitor (IAI) produced central areas of lysis inhibition.
Methods of quantitating IA and IAI were developed. Activator measurements showed :
1. Undiminished IA concentrations in plasma and serum stored for 3 weeks at + 4° C.
2. 60–80% loss of IA during euglobulin precipitation at pH 5.2 and at pH 6.
3. Presence of a plasminogen activator in commercial thrombin preparation. Inhibitor measurements showed:
1. Decrease of IAI in plasma and serum after 4 days storage at +4° C.
2. A physiological decrease of IAI in late pregnancy.
3. That IAI was composed of either larger or more asymmetrical molecules than either plasminogen or IA.
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References
- 1 Bruce S. The effect of calcium on fibrinolysis in vitro. J. clin. Path 1964; 17: 282-286
- 2 Caughey D.E, Highton T.C. Components of the fibrinolytic system in synovial joints. Ann. rheum. Dis 1967; 26: 297-305
- 3 Fearnley G.R, Balmforth G.V, Fearnley E. Evidence of a diurnal fibrinolytic rhythm, with a simple method of measuring natural fibrinolysis. Clin. Sci 1957; 16: 645-650
- 4 Flute P.T. The fibrinolytic System of Human Plasma. Thesis for M. D: University of London; 1959
- 5 Gallimore M.J, Shaw J.T.D. Some aspects of fibrin clot lysis and its inhibition by human serum. Thrombos. Diathes. haemorrh. (Stuttg.) 1967; 18: 101-113
- 6 Heimburger N, Schwick G. Protides of the Biological Fluids. Elsevier Public: New York; 1962: 303-307 (9th Colloquium Bruge, 1961)
- 7 Kowalski E, Kopec M, Niewiarowski S. An evaluation of the euglobulin method for the determination of fibrinolysis. J. clin. Path 1959; 12: 215-218
- 8 Merskey C, Kleiner G.J, Johnson A.J. Quantitative estimation of split products of fibrinogen in human serum; relation to diagnosis and treatment. Blood 1966; 28: 1-18
- 9 Nilsson I.M, Kullander S. Coagulation and fibrinolytic studies during pregnancy. Acta obstet. gynec. scand 1967; 46: 273-285
- 10 Nilsson I.M, Olow B. Fibrinolysis induced by streptokinase in man. Acta chir. scand 2661 123: 247-962
- 11 Permin P.M. Properties of the fibrinokinase-fibrinolysin system. Nature (Lond.) 1947; 160: 571-572
- 12 Todd A.S. Localisation of fibrinolytic activity in tissues. Brit. med. Bull 1964; 20: 210-212
- 13 Walton P.L. An improved fibrin plate method for the assay of plasminogen activators. Clin. chim. Acta 1966; 13: 680-684
- 14 Wolf P, Walton K.W. Investigation of a quantitative anomaly encountered in the assay of fibrinogen by immunodiffusion. Immunology 1965; 8: 6-24
- 15 Wolf P. The measurement of plasminogen (on Type I fibrin agar plates). Thrombos. Diathes. haemorrh. (Stuttg.) 1968; 20: 50